Valuable Yellow Polyphenol Components Uniquely Found in Ashitaba

Valuable Yellow Polyphenol Components Uniquely Found in Ashitaba

When the leaves and stems of Ashitaba are cut, viscous yellow sap comes out from the cut surface. This yellow sap is not found in any other Apiaceae plants, but found exclusively in Ashitaba. Only a few milligrams of this sap can be collected from one Ashitaba stem, which makes it very valuable.

Ashitaba polyphenols are plentifully contained in this yellow sap and are called chalcones. Over 10 types of chalcones are confirmed to exist in the yellow sap.

We are the first manufacturer in Japan which succeeded in powderising the Ashitaba polyphenols in 1999 and, consequently, introduced it as a new functional material. We have been studying its utility and functionality ever since.

What is an Ashitaba Polyphenol “Chalcone”?
Xanthoangelol 4-hydroxiderricin

“Chalcones”, the main components of the Ashitaba polyphenols, are the polyphenols that have yellow flavonoid pigments, which are particularly rich in two types of chalcones, 4-hydroxiderricine and xanthoangelol, and their efficacy has been attracting much attention.

Fat Reduction Mechanism of Ashitaba Polyphenols

A test of Ashitaba polyphenol on rats was conducted and lipid metabolism in liver was analysed from the expression level of mRNA and 4-hydroxiderricin and xanthoangelol were found to have a fat accumulation inhibiting effect and a fat burning promoting effect, respectively.
Sap Collection
  • Collecting yellow sap
  • Cutting roots at 10-15cm height at right angle
  • Yellow sap oozing
  • Collecting yellow sap carefully
  • Filtering

・Anti-metabolic syndrome

  1. An adiponectin production promoting effect
  2. A promoting effect on leptin production in mice
  3. A reduction effect on visceral fat weight as well as an inhibiting effect on body weight gain in mice
  4. A reducing effect on the sectional area of the visceral fat from a human studyFor more detailed information, please click here

An Abdominal Fat Reducing Effect by Taking CHALSAP

Significant reductions in the abdominal total fat and the visceral fat area after 8 weeks of CHALSAP-P8 intake were observed.

Reduction in Abdominal Fat Area by Taking CHALSAP
Reduction in Abdominal Fat Area by Taking CHALSAP

・An anti-bacterial effect

  • An anti-bacterial effect on Helicobacter pylori (in vitro)
  • An anti-bacterial effect on a heat-resistant acidophilic bacterium (Alicyclobacillus sp.)

・An anti-tumour effect

  • An inhibitory effect on a carcinogenesis initiator/carcinogenesis promotor
  • An apoptosis-inducing effect on cancer cells
  • Cancer cytotoxic activityFor more detailed information, please click here

    Ashitaba contains two types of chalcones (xanthoangelol and 4-hydroxiderricin) as its main components. Previously we reported that chalcones were found to have an apoptosis-inducing effect on cancer cells. This time, we filmed the process of a cancer cell (a human liver cancer-derived cell line HepG2) dying out.

    Control (additive-free)
    A cancer cell, once, shrinks before it splits into two cells. This process repeatedly occurs, which leads cancer cells to increase in their number.
    Cancer cells become frothy and die out over a course of several hours when xanthoangelol is attached to them. This phenomenon is typically found in apoptosis (i.e. a suicide death of cells) and the cancer cells decompose their own DNA and, eventually, die out.
    Cancer cells rapidly shrink and break down within one hour when 4-hydroxiderricin is attached. The mechanism of this phenomenon is thought to differ from that of apoptosis.

    (Supervisor) Prof. UTSUMI Toshihiko, Graduate School of Medicine Applied Molecular at Yamaguchi University

・A production enhancing effect on Nerve Growth Factor (NGF、BDNF、GDNF)

・An antioxidant effect

・An immunostimulatory effect from a human study


<Anti-bacterial Activity against Helicobacter Pylori>JP3,958,748

<Anti-bacterial Effect on Heart-resistant Acidophilic Bacterium (Alicyclobacillus sp.)>JP4,643,934

Stability & Safety

Safety of CHALSAP

Acute toxicity test
Rats (males and females) oral administration, CHALSAP-P8
2,000mg/kg/day、OECD423→ No Effect
Ames test
Salmonella typhimurium 4 strain and Escherichia Coli 1 strain and OECD 471
1,000μg/plate as Xanthoangelol and 4-Hydroxyderricin and 12,500μg/plate as CHALSAP-P8
→ Negative(However, an anti-bacterial effect was observed on S. typhimurium TA100 strain)
Chromosomal aberration test
Chinese hamster ovary-derived cell (CHO), OECD 473

Chromosomal aberration:(short time processing method, -S9mix)0.250mg/mL negative
(short time processing method, +S9mix) 0.200mg/mL negative
(18 consecutive hour processing method) 0.075mg/mL negative
(32 consecutive hour processing method)0.050mg/mL negative


Specifications of Ashitaba Polyphenol CHALSAP Products

Ashitaba Polyphenol CHALSAP-P8A powder product produced by freeze-drying a unique extract (chalcones) collected from the stems of Ashitaba. This product has a great concentration of chalcones and long-term storage stability.Ashitaba Polyphenol CHALSAP-P8

yellow powder with a unique smell
Total chalcone content:
8.0+ %

Ashitaba Polyphenol CHALSAP-L3A liquid product produced by emulsifying the unique extract (chalcones) collected from the stems of Ashitaba. This is aqueous-dispersible, heat-resistant and acid-resistant.Ashitaba Polyphenol CHALSAP-L3

yellow opaque liquid with a unique smell
Total Chalcone content:
3.0+ %
Application & Blending Examples

Primary Application(Ashitaba Polyphenol CHALSAP-P8)

  • Capsule
  • Tablet
  • Granule

Primary Application (Ashitaba Polyphenol CHALSAP-L3)

Ashitaba polyphenol is fat-soluble and the range of its application to food has been limited. However by making an aqueous-dispersible liquid product, “CHALSAP-L3”, we have made it possible to apply it to common food such as drink and jelly.

  • Jelly
  • Drink
  • Chocolate
  • Butter