“Chalcones”, the main components of the Ashitaba polyphenols, are the polyphenols that have yellow flavonoid pigments, which are particularly rich in two types of chalcones, 4-hydroxiderricine and xanthoangelol, and their efficacy has been attracting much attention.

・Anti-metabolic syndrome

1. An adiponectin production promoting effect
2. A promoting effect on leptin production in mice
3. A reduction effect on visceral fat weight as well as an inhibiting effect on body weight gain in mice
4. A reducing effect on the sectional area of the visceral fat from a human study

An Abdominal Fat Reducing Effect by Taking CHALSAP

Significant reductions in the abdominal total fat and the visceral fat area after 8 weeks of CHALSAP-P8 intake were observed.

・An anti-bacterial effect

• An anti-bacterial effect on Helicobacter pylori (in vitro)
• An anti-bacterial effect on a heat-resistant acidophilic bacterium (Alicyclobacillus sp.)

・An anti-tumour effect

• An inhibitory effect on a carcinogenesis initiator/carcinogenesis promotor
• An apoptosis-inducing effect on cancer cells
• Cancer cytotoxic activity

  Ashitaba contains two types of chalcones (xanthoangelol and 4-hydroxiderricin) as its main components. Previously we reported that chalcones were found to have an apoptosis-inducing effect on cancer cells. This time, we filmed the process of a cancer cell (a human liver cancer-derived cell line HepG2) dying out.

  Control (additive-free)

  A cancer cell, once, shrinks before it splits into two cells. This process repeatedly occurs, which leads cancer cells to increase in their number.

  Xanthoangelol

  Cancer cells become frothy and die out over a course of several hours when xanthoangelol is attached to them. This phenomenon is typically found in apoptosis (i.e. a suicide death of cells) and the cancer cells decompose their own DNA and, eventually, die out.

  4-hydroxiderricin

  Cancer cells rapidly shrink and break down within one hour when 4-hydroxiderricin is attached. The mechanism of this phenomenon is thought to differ from that of apoptosis.

  (Supervisor) Prof. UTSUMI Toshihiko, Graduate School of Medicine Applied Molecular at Yamaguchi University

・A production enhancing effect on Nerve Growth Factor (NGF、BDNF、GDNF)

・An antioxidant effect

・An immunostimulatory effect from a human study

Acute toxicity test

Rats (males and females) oral administration, CHALSAP-P8
2,000mg/kg/day、OECD423→　No Effect

Ames test

Salmonella typhimurium 4 strain and Escherichia Coli 1 strain and OECD 471
1,000μg/plate as Xanthoangelol and 4-Hydroxyderricin and 12,500μg/plate as CHALSAP-P8
→　Negative（However, an anti-bacterial effect was observed on S. typhimurium TA100 strain）

Chromosomal aberration test

Chinese hamster ovary-derived cell （CHO）, OECD 473

Chromosomal aberration:(short time processing method, －S9mix)0.250mg/mL negative
(short time processing method, ＋S9mix) 0.200mg/mL negative
(18 consecutive hour processing method) 0.075mg/mL negative
(32 consecutive hour processing method)0.050mg/mL negative

Property:

yellow powder with a unique smell

Total chalcone content:

8.0⁺ %

Property:

yellow opaque liquid with a unique smell

Total Chalcone content:

3.0⁺ %

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Ashitaba polyphenol is fat-soluble and the range of its application to food has been limited. However by making an aqueous-dispersible liquid product, “CHALSAP-L3”, we have made it possible to apply it to common food such as drink and jelly.

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